FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49057579
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1084560.22107898965010075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC234900.024.5707284
TTCGACG20100.023.5065146
CAGACCG248200.023.355223
GACCGCG263500.021.8075355
GAACGAT257300.020.83309245
ACCGCGT276600.020.7827846
CCGCGTT284500.020.1334447
CGCGTTC285200.020.0916928
TCAGACC300600.019.6212482
GCGTTCT292650.019.5644639
CTCAGAC343750.018.5352251
AGAACGA298850.018.01170344
ATACGGA305600.017.55316733
AATACGG306600.017.51793532
TACGGAG309000.017.3381834
CAATACG322700.016.82476631
CGTACGC65200.016.7708475
CCGTACG66350.016.5837064
AAGAACG324650.016.55302243
CCCAATA337800.016.2704529
Produced by FastQC (version 0.11.5)