FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49057579
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG585730.11939643413711874No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA519170.10582870385837832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG193400.014.66692945
GTTGTCG24100.014.57629726
GGGAGGT282400.014.1693723
ATAGGGG201100.014.13181938
GGCATAG203900.013.90458235
GGTCACT296600.012.8590837
GTCACTC301150.012.8144248
GGAGGTC310600.012.8133694
GAGCTTA223150.012.73122744
ACTCTCC312900.012.48478511
TGGCATA229250.012.39851234
CATAGGG241250.011.74275137
CACTCTC338300.011.467810
GAGGTCA354300.011.3162085
GCATAGG252800.011.25083136
TCACTCT362450.011.0505529
TGGGAGG284700.010.8326772
AGGTCAC370500.010.8265296
GCGTTCG18450.010.36296215
GCTCTGT379800.010.24131222
Produced by FastQC (version 0.11.5)