FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45549003
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG51900.016.30053944
GACCGCG112100.016.197415
AGACCGC114050.015.9796554
ACCGCGT116450.015.6116766
CAGACCG119900.015.3312873
CGTTACG17150.015.21832221
CCGCGTT121550.014.9566417
CGCGTTC121750.014.9135918
CCGTACG57900.014.7666754
GAACGAT115350.014.70744245
AACGCTA68150.014.65851623
ACGCTAC69400.014.45933424
CGTACGC58250.014.4075645
GTCCGTT36450.014.3941851
AGACGCT162000.014.1247841
TCGGCGC60200.014.01580845
CCCCGCA151200.013.88362235
CCGCAGA169400.013.6538637
CCCCGCT74400.013.6499881
AAGCGGC156300.013.61774228
Produced by FastQC (version 0.11.5)