FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48905514
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3880200.7934074673052204No Hit
GTTTAAACAGTTCAGTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCT490230.10024023058013458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG32100.014.92987626
GGGAGGT206050.013.1035893
CGTAACG21900.012.94529942
GGAGGTC215400.012.7959354
ATAGGGG167850.012.66753538
GGCATAG177400.012.13779935
GAGGTCA233900.011.9954875
GTCACTC231800.011.5994168
GGTCACT237350.011.3661027
AGCTTAG205200.011.08560645
CACCGAA261000.011.07766545
GAGCTTA196600.010.84951844
TGTCGTT46500.010.83868728
AGGTCAC262150.010.7886496
TGGCATA205350.010.748734
GCACCGA265600.010.74179444
TACGGTT33350.010.38978335
CATAGGG213800.010.12392737
ATATAGC59600.010.1174296
ACTCTCC270550.010.10439611
Produced by FastQC (version 0.11.5)