FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46072418
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1312500.28487760290766595No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT999440.21692805443812394No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG679520.1474895456973845No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT481140.10443124560990048No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC473110.10268833730411112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC223650.021.1697834
GACCGCG227850.020.7597895
CAGACCG230050.020.6983783
ACCGCGT234650.020.2127846
GAACGAT236550.020.01081845
CCGCGTT247250.019.1827287
CGCGTTC250600.018.9352688
CCGGCTT397200.018.9347382
GCCGGCT350250.018.8097631
TTCACGC400350.017.8631237
TCACGCT401450.017.8029738
TCAGACC275850.017.4576932
CGGCTTC459100.017.0147423
AGAACGA282250.016.89961644
ACGCTCA448800.016.15013310
CCGTACG84550.016.1440184
CTCAGAC326350.016.0296551
AATACGG298050.015.88895132
ATACGGA304750.015.58979733
CACGCTC459600.015.5504869
Produced by FastQC (version 0.11.5)