FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46072418
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA860840.1868449795710744No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG816660.1772557281451996No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG809440.17568863001720467No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG510930.1108971532598962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT329500.016.1444913
GGTCACT351600.014.7243537
GTCACTC356000.014.5795878
GGAGGTC370450.014.2198844
TGGGAGG292300.013.8958742
TCACTCT397400.013.2740369
GAGGTCA410500.013.1490125
CTGGGAG347150.012.62828351
ACTCTCC411250.012.62012911
AGCTTAG305400.012.61360945
GGCATAG310700.012.3418735
TCAGACG106000.012.3341082
AGGGATC211650.012.3013452
GTTGTCG25450.012.28784126
ATAGGGG318200.012.14576938
TGGCATA327600.011.81510634
GAGCTTA338000.011.42250544
TCTCCCC460550.011.37112413
TAGCGTA7550.011.32902237
CACTCTC462500.011.20400110
Produced by FastQC (version 0.11.5)