FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46078184
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG481260.10444422028437579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG40450.020.912796
CGGTATA56550.018.45954320
CGACGAG44750.017.4955648
TCGACGA49250.017.221777
CGTTACG19950.017.02823621
GTTCGAC47200.016.875215
GTCCGTT37450.015.7523651
GCCGGCT170650.015.4902381
CCGGCTT200600.015.1760612
AACGACG29900.014.97336416
TCACGCT181600.014.5691178
TTCACGC183800.014.5416487
AACGCTA177850.014.18042423
AGACCGC119450.014.1462634
CAGACCG122300.014.0005843
ACGCTAC180450.013.98857524
ACCGCGT121950.013.9101636
GACCGCG122100.013.8392385
CGCTACC187650.013.43985425
CCGCGTT128500.013.236147
Produced by FastQC (version 0.11.5)