FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46078184
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG683370.1483066259729333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG243000.013.56851445
GGGAGGT356500.013.4861073
GGAGGTC363200.013.1072184
GGTCACT363450.012.8382047
ATAGGGG267450.012.41019738
GTCACTC376500.012.3693118
GAGGTCA385900.012.3128715
GGCATAG270100.012.2466935
GTTGTCG29600.012.00948626
ACTCTCC408500.011.50499811
TGGCATA288750.011.4790234
GAGCTTA287300.011.47635244
TCACTCT428200.011.0965369
CACTCTC427500.010.94102710
TGGGAGG335600.010.4379312
TAATCGG40900.010.1762814
CTGGGAG369700.010.102021
CATAGGG345400.09.76583837
AGGTCAC511700.09.545196
GCATAGG354300.09.48869236
Produced by FastQC (version 0.11.5)