FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46378349
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT813280.17535768683788205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC176600.023.6332684
ACCGCGT181000.022.8963456
CAGACCG187500.022.4874273
GACCGCG186300.022.2328955
CCGCGTT192300.021.60947
CGCGTTC195650.021.2158018
TCAGACC225100.019.0852952
GCGTTCT220150.018.9569369
GAACGAT220300.017.89214945
CTCAGAC283300.017.2733021
CGTTCTC268350.015.97953810
AGAACGA251650.015.69000544
GTCCGTT30400.015.2741041
AATACGG270300.015.14952932
ATACGGA271800.015.10664633
TACGGAG270550.015.00991434
TTCGACG12200.014.7531676
GCACTAC74950.014.74081725
CGTTACG16450.014.3626721
CAATACG285850.014.34114931
Produced by FastQC (version 0.11.5)