FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43207167
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG484100.11204159717298752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG31100.028.1338396
TCGACGA39500.022.3217757
GTTCGAC39500.022.0940025
CGACGAG39850.021.5612938
CGGTATA55750.020.963220
TTCACGC214450.018.837367
GCCGGCT148250.018.4924321
CGTTACG19900.017.88697421
TCACGCT225300.017.8403428
CCGGCTT174350.017.5193352
GCCGGTT74650.017.337221
CCGGTTT82250.016.2985942
AGTTCGA56600.016.0150554
ATGACGG72000.015.71358416
ACGCTCA260900.015.61292210
GACGGTA76400.015.40409218
ACGCTAC259900.015.13505224
AACGCTA260200.015.108891523
CCCGTAT29650.014.8030551
CCCGGAT108200.014.6865191
Produced by FastQC (version 0.11.5)