FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51789534
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG741000.14307910165787552No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG678200.13095309952006903No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA663690.1281513751407765No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG606370.11708350185193787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG35000.017.03307526
GGGAGGT361700.014.7538193
GGAGGTC357300.014.6768824
AGCTTAG249800.014.46207345
ATAGGGG267100.013.63023838
GGCATAG268200.013.38840435
GAGCTTA289550.012.46353944
TGTCGTT48000.012.4202528
TGGCATA295600.012.23111734
ATATAGC55500.012.122866
GGTCACT429350.012.0854317
GTCGTTC49900.011.99240829
ACTCTCC441000.011.87831811
GCTAAGT53500.011.69234414
TGGGAGG326750.011.6421862
GTCACTC456550.011.5478078
TCGTTCC54350.011.3826630
CACTCTC462150.011.33961610
AGGGATC184650.010.9346912
GAGGTCA492650.010.9085875
Produced by FastQC (version 0.11.5)