FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47659677
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT897670.18834999658096716No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG661570.13881126386987475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC220550.020.8708084
CAGACCG226550.020.4173873
GACCGCG230250.019.8250775
ACCGCGT237000.019.3645536
CCGCGTT252050.018.1993437
TTCGACG15350.017.8803966
CGCGTTC257450.017.8176148
TCAGACC277250.017.3339392
GAACGAT268800.017.25782245
GCCGGCT216100.017.1970161
CGTTACG28300.016.61807621
TCACGCT239800.016.4928198
CCGGCTT255400.016.2797832
GTCCGTT33900.016.2406861
GCGTTCT288100.015.9220579
TTCACGC251250.015.8576097
CTCAGAC329000.015.7741581
ACGCTCA268400.015.17962810
AGAACGA316500.014.73511544
AACGCTA260750.014.51527623
Produced by FastQC (version 0.11.5)