FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54170298
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1028690.189899269152996No Hit
CCCATGTCTGCCTGCTGTGACCTGCAGCTCCTCGTACTTGGTCTGGTACCA843790.15576617282038951No Hit
CTCCAGGTTCTGCCTCACAGTCTTGGTGCCCTGCTCCTGCAGCAGGGTCCA636870.11756811823335363No Hit
CCCCACGATGCTGTCCAGCTGCCTCCTGAGGTTGTTGATGTACTGCTCGAA622680.11494860153806058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACGC263350.021.3697037
ACGCTCA275150.020.82363110
AACGCTA268750.020.64689623
ACGCTAC269400.020.56370224
CGCTACC275150.020.07843225
AAAACGC285050.019.5003421
GAAAACG291550.018.99611720
AAACGCT299200.018.6132922
GACCGCG86700.018.2041075
AGACCGC86600.018.0691364
TCACGCT312800.018.0439228
ACCGCGT88950.017.844226
CCGGCTT382050.017.7915332
CAGACCG95250.016.7591383
CACGCTC348050.016.3392629
CCCGTAT31050.016.3153111
CCGCGTT96600.016.2758837
CGCTCAG366700.015.77895811
CGCGTTC101700.015.4394398
CGTTACG26750.015.05897821
Produced by FastQC (version 0.11.5)