FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47428252
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG119000.014.38866845
GGCATAG125850.013.51547435
ATAGGGG134550.012.99307538
ACCCGGC77350.012.5662555
GAGCTTA143850.011.96562544
CATAGGG147400.011.73800737
ATAGCTC77700.011.3511739
ACTCTCC236000.011.28768111
GGGAGGT257800.011.0800833
GGTCACT236500.011.0444167
TGGCATA155200.011.01729634
GTCACTC243150.010.9368378
GCATAGG158300.010.84430636
TCAGACG83400.010.7424032
TCACCCG99400.010.6659323
TAGCTCT87700.010.21090140
CACTCTC269900.09.94465610
AGCATAG89350.09.59430145
TGATAGC98550.09.54300437
CCGGACA129900.09.43966312
Produced by FastQC (version 0.11.5)