FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48008076
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1193950.24869773993858868No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT587410.12235649685273786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG35850.024.6642916
GCCGGCT265250.024.4221611
CCGGCTT323800.022.7925782
TCGACGA41750.021.5021387
TTCACGC343650.021.147137
CGTTACG26400.020.87987321
TCACGCT346650.020.879748
CGGCTTC370450.020.3838253
CGGTATA57600.020.23357220
AACGCTA357150.019.9635723
ACGCTAC358850.019.86276624
GTTCGAC44450.019.488495
CGCTACC368650.019.31675725
ACGCTCA381850.019.28494810
CACGCTC384750.018.9115289
AAAACGC380700.018.7699621
CGACGAG45650.018.6794348
GAAAACG383150.018.62055820
AAACGCT386700.018.50781622
CGCTCAG462450.016.03082511
Produced by FastQC (version 0.11.5)