FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44153206
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG466820.10572731683402559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG150650.015.17255545
ATAGGGG157550.014.651175538
GGCATAG158550.014.51623135
GGGAGGT234650.013.963873
GAGCTTA174600.013.11715544
GGAGGTC260650.012.6043444
TGGCATA182650.012.60072334
GTTGTCG18350.012.261312526
GGTCACT266350.012.0105347
CATAGGG195100.011.77364637
GTCACTC273200.011.594118
GCATAGG206500.011.11277836
TGGGAGG226000.010.9669122
ACTCTCC307850.010.35490511
TAGGGGA318850.09.87816539
TCAGACG61250.09.7853342
AGGTCAC352800.09.6731856
AGGGGAG332050.09.587172540
TCACTCT347350.09.5659629
TCTCCCC338800.09.56176313
Produced by FastQC (version 0.11.5)