FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46982660
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG840020.1787936230089995No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT679400.1446065420731819No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG535270.11392926666987352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC189400.021.7515684
CAGACCG197950.020.8916233
GACCGCG198300.020.6051275
ACCGCGT209350.019.5920226
CCGCGTT226650.018.1164327
CCGGCTT328600.017.8373452
GCCGGCT285900.017.7469731
CGCGTTC188150.017.5783718
CGTTACG18600.017.54249221
TCACGCT331400.017.3869198
TTCACGC331750.017.314327
TCAGACC252300.016.980082
CTCAGAC293000.016.0882321
CGCGTTT50700.015.7981998
CTACACG206550.015.6420945
GTCCGTT36950.015.588971
CGGCTTC395000.015.4708743
GCGTTCT215050.015.4423179
ACGCTCA376950.015.36947410
CCGTACG49250.014.6192784
Produced by FastQC (version 0.11.5)