FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51064773
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT746340.14615555032429106No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG682360.13362636508733722No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA649410.12717377594139115No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG517560.10135362787180117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG51900.026.2757076
GTTCGAC54750.024.3743675
CGGTATA83900.022.20634720
TCGACGA62750.021.7630797
CGACGAG62200.021.5201028
ACCGCGT180600.020.435036
GACCGCG183550.020.1072315
AGACCGC185050.020.078314
CAGACCG190550.019.5460133
CCGCGTT199100.018.5431147
CGCGTTC199500.018.4483768
AGTTCGA74400.018.3302694
CGTTACG26950.018.28503421
GCACTAC101850.018.06750125
CCCGTAT42550.017.2419831
GTCCGTT42900.017.2062281
ATGACGG105950.016.99045416
GCCGGCT215800.016.372591
GACGAGG105100.016.3534139
CCGGCTT263650.016.3118082
Produced by FastQC (version 0.11.5)