FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45615012
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGG465430.10203439166035953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG135450.014.76451445
GGCATAG137950.014.52946235
GTCAGGA186550.014.3219541
ATAGGGG145050.013.9584438
GGGAGGT242450.013.334983
TGGCATA153750.013.16801834
GAGCTTA152650.013.11554244
GTTGTCG17800.013.01812326
GGAGGTC250000.012.4192524
CATAGGG166200.012.15506837
AGTTAGG41200.011.8500164
ACCCGGC65850.011.5135335
GCATAGG180750.011.18905736
ATAGCTC65350.010.49931839
TCAGACG74200.010.3748572
AGGGATC116750.010.2869442
GCTCTGT295800.010.22154522
TCTCCCC299950.010.16965613
TGTCCAA304650.010.08004926
TATCCTA77250.010.0474325
Produced by FastQC (version 0.11.5)