FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51342840
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2446870.47657472784910226No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1053270.20514447584122736No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA516850.1006664220366462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC473850.025.7258344
ACCGCGT475250.025.626336
GACCGCG475450.025.5871545
CAGACCG483650.025.2698613
GAACGAT484500.025.04480745
CCGCGTT488950.024.8893597
CGCGTTC482600.024.8626588
TCAGACC518150.023.8175752
GCGTTCT537900.022.3569229
CTCAGAC585750.021.8638551
AGAACGA558650.021.74914644
CGTTCTC600600.020.30056610
TTCGGCG93050.020.21520444
AATACGG600700.020.14040232
ATACGGA604000.019.9668633
TACGGAG604250.019.8763734
TCGGCGC94900.019.84450545
ACTCCCC619000.019.81401625
CAATACG615300.019.71408531
AAGAACG619400.019.59072343
Produced by FastQC (version 0.11.5)