FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45233019
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT513010.11341493699547228No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG453180.10018787381845992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC135000.022.016614
ACCGCGT141450.020.9629576
CAGACCG146050.020.4124983
GACCGCG148850.019.877345
CCGCGTT150250.019.7056227
CGCGTTC136850.018.6594098
GAACGAT171100.017.33081445
TCAGACC180450.016.9957942
TCGGCGC76550.015.87091945
TTCGGCG78200.015.70867144
CCGTACG80850.015.4452284
ACGTTCG17050.015.43837456
CGTTACG12600.015.35599921
GCGTTCT167900.015.2622959
AGAACGA196750.015.11716244
CTCAGAC227000.014.9773591
CGTACGC83450.014.9100875
AATACGG200750.014.78196832
ATACGGA202050.014.77593833
TACGGAG200350.014.75518634
Produced by FastQC (version 0.11.5)