FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45233019
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC87150.015.0247255
TCACCCG96700.013.8908453
AGCTTAG153500.013.69932945
GGCATAG156950.013.29803735
GGGAGGT285550.012.6702363
CACCCGG110200.012.3113854
GAGCTTA176450.012.04569144
GGAGGTC266800.011.890624
TGGCATA177200.011.87990634
ATAGGGG183250.011.8313838
CCGGACA112500.011.59205512
ACTCTCC277100.011.27033211
CGGACAG111050.011.24456619
TAGCGTA11400.011.04835337
CATAGGG195150.010.93734637
ATAGCTC111300.010.79045439
ACGGACA119200.010.72159218
GGTCACT297000.010.30217557
GCATAGG207450.010.29969936
ACACGGA122250.010.26974716
Produced by FastQC (version 0.11.5)