FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52912428
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT778090.14705240893500485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG31050.025.7252066
GAACGAT158250.023.77254545
AGACCGC178600.020.9888464
CGGTATA50550.020.60773320
CAGACCG185000.020.4451833
ATACGGA204700.018.3664733
CGCAAAC66200.018.3532425
AGAACGA206050.018.32324844
GTTCGAC43650.018.2994425
TCGACGA44850.018.2615327
TACGGAG208600.018.00153534
AATACGG209300.017.99504532
CGACGAG44450.017.9703668
AACGCTA110300.017.52244223
AAGAACG215600.017.52205543
ACGCTAC112650.017.1369324
CGTACGC69950.017.0483325
CCGTACG69450.016.976734
CAATACG224650.016.81554631
GACCGCG225500.016.7631865
Produced by FastQC (version 0.11.5)