FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52912428
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG156400.014.70184545
ATAGGGG157150.014.49136338
GGCATAG162750.013.95042535
GGGAGGT241750.012.8890253
CATAGGG182050.012.48425837
GAGCTTA184200.012.44755844
GGTCACT239150.012.4186277
GTCACTC244400.012.3545318
GCATAGG189800.012.02150936
ACTCTCC254050.011.9216211
TGGCATA195450.011.75449634
GTTGTCG24400.011.52555526
GGAGGTC292550.010.6970444
GAGGTCA293000.010.5889465
TCACTCT299800.010.4025799
CACTCTC292950.010.30017810
ACACTCG111750.09.6432794
CACTCGA108700.09.6245295
TCAGACG85650.09.6133622
TGGGAGG259450.09.5900972
Produced by FastQC (version 0.11.5)