FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48114158
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT916990.19058631349217417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT175950.025.47165945
AGACCGC201750.022.5721594
TTCGACG16700.021.9611666
CAGACCG211850.021.6554473
AGAACGA218500.020.52166444
ACCGCGT226050.020.1460676
GACCGCG231400.019.6414955
CCGCGTT232950.019.5391717
CGCGTTC233100.019.5265778
ATACGGA245100.018.5795733
TCAGACC257200.018.335772
TACGGAG255450.017.83537334
AATACGG257500.017.70268832
AAGAACG254100.017.68200343
CAATACG259600.017.60344331
GCGTTCT260500.017.5331129
TCGACGA20050.017.3936337
ACGGAGA262050.017.32583435
CGGAGAG273300.016.62912436
CCGCTTA97050.015.76418243
Produced by FastQC (version 0.11.5)