FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48114158
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCT655760.13629252329428687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGGA327850.015.6328671
AGCTTAG132600.013.92915245
ATAGGGG140100.013.24860638
GGCATAG137100.013.16120935
CGGGACG80650.012.69397317
AGCGCTC63950.012.5242739
ACGTCTC82950.012.47845621
TCTCGGG93050.011.72708614
GAGCTTA158900.011.60970944
GACGTCT91400.011.44764620
CGCTCTC69350.011.41940211
CATAGGG163400.011.37313337
GGGAGGT233250.011.3651943
TGGCATA160850.011.3297834
GCGCTCT71250.011.14610210
TCAGACG69400.010.7350922
GGGACGT100550.010.36082618
TCGGGAC103800.010.31749716
CGTGGCG111550.010.2706751
ACACAGC507750.010.217637
Produced by FastQC (version 0.11.5)