FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45752348
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT536620.11728796957043604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG24800.020.0434386
AGACCGC139400.018.5561584
CAGACCG143450.018.1577073
GAACGAT145450.017.6438445
GACCGCG148350.017.3760475
CCGCGTT149200.017.2610937
ACCGCGT150550.017.1361926
CGCGTTC150800.017.1226988
CCGTACG45500.016.9070454
CGTACGC45750.016.8638575
CGGTATA38250.016.58245520
CTCCGTT65350.014.7368611
CGACGAG30700.014.5796248
TACGCCA55250.014.3298627
GTTCGAC30650.014.3106075
GTCCGTT42000.014.3043721
AATACGG181400.014.13603732
AGAACGA181650.014.12769944
ATACGGA181850.014.11376533
TCAGACC189050.014.0994682
Produced by FastQC (version 0.11.5)