FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45752348
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG106450.012.87490738
AGCTTAG113000.012.027801545
GGCATAG113800.011.93185635
GTTGTCG21150.011.60659526
CATAGGG121750.011.17460937
GCATAGG130300.010.49233536
GTCACTC183150.010.2448178
GGTCACT177600.010.2339597
GGGAGGT195000.010.1666293
GAGCTTA137000.010.12063844
TGGCATA136800.010.06519334
ACTCTCC200050.09.39240811
ACACTCG93750.09.3265574
CACTCGA89450.09.32055955
GGAGGTC204950.09.2014674
TAATCGG35250.09.0546624
GAGGTCA227450.08.8229525
TCACTCT220400.08.6976719
CACTCTC212450.08.65292710
ACTCGAG107000.08.608926
Produced by FastQC (version 0.11.5)