FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50050064
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT45150.017.0582331
CCCGTAT30350.015.2853081
CCCGGAT142650.014.8395841
CCGGATA96400.014.77471352
CGGATAC84800.014.6996113
CGTTACG20050.014.47581421
GCGGACT61800.014.235762
CCGTACG53050.014.1661884
AACGCTA121450.014.09796723
ACGCTAC123800.013.88487824
GCCGGCT139550.013.7329991
CGTACGC56650.013.6234215
CGCTACC126700.013.4960825
TTCGCCA86700.013.49436114
CTCCGAT44700.013.149181
GTTTCGC90900.013.11837612
AGTATAC88200.012.83161634
TTCACGC148400.012.7960327
CCGGCTT167950.012.7540852
CTCCGGT72150.012.7347231
Produced by FastQC (version 0.11.5)