FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52907527
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG114300.012.53918738
AGCTTAG112500.012.45970245
GGCATAG115900.012.13308235
CATAGGG128350.011.25421937
GGGAGGT205300.011.0804173
GAGCTTA128450.010.93006144
GCATAGG136200.010.45685736
GTTGTCG15900.010.32994226
TGGCATA147300.09.77578734
ACTCTCC221550.09.64770311
TAGGCTA72200.09.6292710
CGTAGGG64500.09.45335137
GCGTAGG65150.09.4280736
TATCCTA84300.09.3948735
GGCGTAG67050.09.12734935
TTACGCG11550.08.960875535
ATCCTAG96900.08.8000986
ACCCGGC68400.08.716975
CGTCAGT76100.08.6697911
GGAGGTC256500.08.6668934
Produced by FastQC (version 0.11.5)