FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49612792
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG164250.012.59274545
GGGAGGT250250.012.370233
AGGTCAC256150.011.724676
GTCACTC250600.011.6343138
GGTCACT254900.011.4556197
ATAGGGG183500.011.35549338
GGCATAG183300.011.19548335
GAGCTTA193200.010.76390644
ACTCTCC277750.010.57809911
GAGGTCA300500.010.398975
GGAGGTC300650.010.2516364
TCACTCT299450.09.9918089
CACTCTC299000.09.79621510
GTTGTCG17750.09.50662926
TAGCGTA5252.0008883E-119.42954937
ACAGACT200150.09.2734052
TCAGACG80550.09.2169922
CATAGGG228650.09.20169737
TGGCATA227250.09.18870934
GCTCTGT337500.08.86655722
Produced by FastQC (version 0.11.5)