FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45256384
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG18450.012.68072426
ATAGGGG96000.010.96916238
GGCATAG97250.010.64241435
AGCTTAG98200.010.56252145
ACCCGGC57950.09.90681655
GGGAGGT178750.09.6744343
ACTCTCC180250.08.97396211
CATAGGG115800.08.89930837
GAGCTTA119050.08.78770244
GCATAGG118100.08.74528836
GGCGTAG58100.08.71321335
CGTAGGG58400.08.66937
GCGTAGG58200.08.66036936
GTCACTC185700.08.5889198
TAATCGG33900.08.5674354
GGTCACT182950.08.5461227
GGAGGTC198850.08.4690914
CACTCGA73800.08.3892625
ACACTCG77200.08.3408364
TTAACGG31050.08.2671083
Produced by FastQC (version 0.11.5)