FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48588688
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT950170.19555374699559697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC167200.026.4200384
CAGACCG171650.025.9317843
GAACGAT172900.025.48148245
GACCGCG184050.024.0011795
TTCGACG34200.023.7535886
ACCGCGT189600.023.2986136
CCGCGTT199050.022.2143337
CGGTATA44000.022.08849120
CGCGTTC202050.021.9048568
ATACGGA205600.021.4806733
TACGGAG205350.021.4416634
AATACGG207700.021.27444832
AGAACGA209650.021.2078844
TTCGGCG77850.020.02994744
TCAGACC228500.020.0212522
TCGACGA40050.019.9461357
GCGTTCT223050.019.9414589
CAATACG223150.019.88176231
CTCAGAC266850.019.2412091
CGACGAG38100.019.016298
Produced by FastQC (version 0.11.5)