FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48588688
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC74200.011.794815
GGCATAG88800.011.72978535
CGAATCG36850.010.864613
CATAGGG96350.010.64753337
GCATAGG97500.010.5911736
TCACCCG91700.010.1300683
GAGCTTA106350.09.94043544
GTCACTC150750.09.6736048
AACTCTA95550.09.6272645
ATAGGGG112400.09.54732538
CGGCCCG93850.09.4478418
CGTAACG17200.09.41599242
TCAGACG86700.09.39055252
GGTCACT149300.09.34369857
CACCCGG100800.09.21688654
TAATCGG33000.09.2026844
AGCTTAG113450.09.17881645
CCGGACA111100.09.03551212
GTAACGA32850.08.97176918
GGGAGGT182300.08.969733
Produced by FastQC (version 0.11.5)