FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45365585
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTGTCCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCC1234160.27204763258315745No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1072020.23630688329049432No Hit
GTCCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC1008820.22237561799324312No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTC994920.21931162135349958No Hit
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTC982820.2166444012570322No Hit
CCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG902600.19896139331169213No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTATAGAGGAAGAAGG893210.19689154234426823No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG848800.1871021832959941No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAA801090.17658540058504701No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT765440.1687270207140501No Hit
CCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTATAGAGGAAGAAG759700.16746174440382505No Hit
CTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGG748920.16508549377242682No Hit
GTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTTCCC699290.15414548274865186No Hit
TTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGC574100.12654967416379617No Hit
CCCCACTTTGCAAACTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCC567160.12501988015805374No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCC537930.11857666995807506No Hit
CCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGC498310.1098431773777413No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT496160.10936924983993923No Hit
GTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTG491060.10824504963399016No Hit
CGTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCC468510.10327432127239183No Hit
CCACGCTGCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGG464850.10246754230106368No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATG458500.10106780282895063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG28700.026.3350776
CGGTATA41850.025.50490420
TCGACGA31450.024.2469027
GTTCGAC35050.021.9490265
CGACGAG35200.021.8554958
AGACCGC203950.019.3015214
ATGACGG53300.019.0760716
CCGGCTT325350.019.0548532
GTATAGA60100.018.66283422
CAGACCG212850.018.5684363
CGTTACG11200.018.50080521
ACCGCGT215250.018.2777966
TCGGCGC84100.017.8944345
TTCACGC344100.017.820447
AGTTCGA44300.017.7214364
CGGCTTC363900.017.7162323
CCGCGTT226350.017.4112837
GACCGCG226350.017.3516565
CGCGTTC229700.017.147568
GTTATAA129450.017.11703940
Produced by FastQC (version 0.11.5)