FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51708461
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3524940.6816950131236743No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG2138610.41358995387621383No Hit
GCCGGTTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1355280.26210023926258413No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1070500.20702608031594674No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA1066380.20622930549025623No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGT1041580.20143318518027445No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG799450.15460719281511784No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTA769420.14879963261718424No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT768860.1486913331263137No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC739520.14301721337248852No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG707390.13680353008379034No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAG657680.12719001634954868No Hit
CTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATTT541300.10468306144327133No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG539180.1042730705135471No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC523850.10130837195096562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT382850.027.6860711
TCACGCT667750.027.3176338
TTCACGC687650.026.540627
GAACGAT653850.025.8665245
AACGCTA711600.025.35591523
ACGCTAC716200.025.19627424
ACGCTCA738700.025.0684210
CCGGCTT479700.025.0247042
CACGCTC739300.024.7985699
AAAACGC730550.024.7666221
CGCTACC730200.024.6664125
GAAAACG737050.024.56347320
GCCGGTT275150.024.5146581
AAACGCT743900.024.34264822
CCGGTTT283750.023.9618072
AGACCGC739650.023.0662424
CAGACCG748050.022.8222753
CGGCTTC531250.022.7786543
GACCGCG757300.022.5197075
ACCGCGT757550.022.5143996
Produced by FastQC (version 0.11.5)