FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52683960
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1160320.22024160674330479No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG959000.18202883761964742No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG587390.11149313756976507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG32050.024.9189746
GACCGCG270400.021.7097575
ACCGCGT272100.021.5960566
AGACCGC275650.021.3860634
CAGACCG285750.020.7403983
TCGACGA39850.020.6624287
CCGCGTT294200.019.9737267
CGCGTTC296250.019.840968
GTTCGAC41000.019.5368125
GTTCCTA116000.018.64826815
TCAGACC328650.018.2658442
CGACGAG46850.017.669328
CGGTATA60850.017.46054620
GCGTTCT349400.016.9129269
CTCAGAC394300.016.8452381
CGTTACG26100.016.37702821
CCGGCTT359250.016.296642
GCCGGCT314150.015.8357721
CCTACTC140150.015.65986218
TTCACGC363850.015.3464537
Produced by FastQC (version 0.11.5)