FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54002904
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT598440.11081626276986882No Hit
CTCGCACCGAGACTCAAGGCCGTCTCCACAACTCCAACCAGTGCAAATGAC586390.10858490128604935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC181000.020.3042964
GACCGCG183550.019.8992925
ACCGCGT186750.019.6058066
CAGACCG196250.018.8411853
CCGCGTT196950.018.5857967
CGCGTTC179150.017.9572688
CGTTACG16300.015.87498521
CGAACGA44100.015.4606932
TCAGACC246050.015.4576552
GCGTTCT215250.015.0187799
ACGAACG46650.014.85675131
CGCACCG197300.014.4976893
CTCAGAC296900.014.1594691
CGCGTTT33350.013.8960848
CCGTACG43600.013.8333894
CCCGTAT39850.013.72328951
CGTACGC42600.013.5767865
GTCCGTT36450.013.3980791
CTCCGAT50800.013.3346951
ACCGAGA224450.013.044146
Produced by FastQC (version 0.11.5)