FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52213780
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1231970.23594729207500395No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG948040.18156892682353204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT248350.023.82702445
AACGCTA248000.021.66877223
ACGCTAC237450.021.56158824
TTCGACG26400.020.9521586
AAAACGC264000.020.48991821
CGCTACC247700.020.47921225
TTCACGC276100.020.2690817
CCGGCTT290000.020.1824442
AGACCGC296450.020.1377374
GAAAACG271000.019.95832420
TCACGCT280200.019.7579968
CAGACCG306650.019.5925563
GCCGGCT312350.019.4084551
AAACGCT280900.019.28919422
ACCGCGT314550.019.0075536
AGAACGA317800.018.7071244
GACCGCG321050.018.629735
CGGCTTC320900.018.4632643
CCGCGTT326850.018.2775767
CGCGTTC327550.018.2267788
Produced by FastQC (version 0.11.5)