FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52213780
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG744730.14263092999587465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG248900.015.66504545
GGGAGGT399100.014.690043
GGCATAG271550.014.21903635
ATAGGGG283000.013.86642238
GGAGGTC417600.013.7160234
GAGCTTA289550.013.48268944
GGTCACT415100.013.3120787
TGGCATA296300.013.09966434
GTCACTC448000.012.6382738
TGGGAGG337950.012.2088362
CATAGGG336600.011.66502537
GTTGTCG28700.011.36462926
ACTCTCC506000.011.309381511
CTGGGAG399350.011.1285291
GCATAGG352100.011.07478236
AGCATAG159750.010.72389345
GCTCTGT538000.010.5561322
TGTCCAA540300.010.49975826
GAGGTCA553650.010.4593225
TAGGGGA536150.010.42484539
Produced by FastQC (version 0.11.5)