FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51996908
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG868700.1670676264057855No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT560880.10786795245594219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG155200.019.9526545
AGACCGC157600.019.6630864
ACCGCGT160500.019.3890616
CAGACCG167350.018.759553
CCGCGTT174850.017.8190577
CGCGTTC178950.017.3841848
CCCGTAT38000.017.2931141
GCCGGCT280350.016.407951
CCGGCTT319500.016.1442452
TCACGCT310950.015.5601478
TTCACGC314500.015.3428947
GTCCGTT41100.015.1674271
CGGCTTC357800.014.799793
TCAGACC218250.014.6938152
ACGCTCA344450.014.5645710
CGTTACG18200.013.96773621
AACGCTA334900.013.92376823
ACGCTAC337350.013.82267324
CACGCTC351000.013.759069
GCGTTCT229650.013.6442139
Produced by FastQC (version 0.11.5)