FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51996908
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA634580.12204187218209206No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG593200.11408370666963505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT274900.013.0894773
AGCTTAG197100.012.86488945
ATAGGGG206300.012.498238
GGCATAG205800.012.34271335
GGAGGTC295300.012.18522554
GGTCACT287350.011.9740387
GTCACTC300950.011.5151598
GAGCTTA228150.011.20269444
ACTCTCC321000.010.9692911
TGGCATA234900.010.90944934
GTTGTCG26750.010.85017626
CATAGGG245600.010.31506837
GCATAGG257250.09.97037636
TAGGGGA341850.09.95784739
AGGGATC190100.09.9555332
CACTCTC363300.09.68612710
TGGGAGG288800.09.5375072
TCACTCT385650.09.3641799
CTGGGAG342850.09.2154761
CGTGGCG115250.08.9233941
Produced by FastQC (version 0.11.5)