FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44790842
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG18950.018.7609986
AACGCTA85600.017.87330623
AGACCGC83900.017.5127934
ACGCTAC80600.017.0559524
GTTCCTA84750.016.80410215
CAGACCG92000.016.2888573
CGCTACC85200.016.24068325
CCTACTC89550.016.02903418
GAACGAT94750.015.74118745
TTCGGCG69900.015.54611144
CCGTACG70900.015.519114
GTCCGTT35500.015.411451
CGTACGC72500.015.1145475
AAAACGC104100.014.80503921
CTCCGAT44600.014.7910271
CGCAAAC46200.014.70732725
GAAAACG107200.014.43976120
CCCGGAT112150.014.4342811
AGTTACC105500.014.11746934
TACGCCA79800.013.9003387
Produced by FastQC (version 0.11.5)