FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44790842
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG127950.014.33149545
ATAGGGG139250.013.41117438
GGCATAG137000.013.38572135
GGGAGGT217000.012.0812593
GTTGTCG19600.012.05304526
CATAGGG158950.011.70654937
GGTCACT211750.011.666597
GAGCTTA160800.011.57160844
GTCACTC217000.011.4465438
TGGCATA162600.011.278316534
GCATAGG165550.011.25345936
ACTCTCC227300.011.02683211
GGAGGTC242300.010.7175014
GAGGTCA271550.09.7780885
CACTCTC251600.09.75616510
TAGCGTA3704.600588E-89.72976137
TCACTCT264450.09.681979
TAGGGGA274100.08.955482539
CACTCGA89050.08.8694135
GCTCTGT280850.08.85221422
Produced by FastQC (version 0.11.5)