FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45793776
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG537720.11742207063248071No Hit
CTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGA468830.10237854157298583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG179950.014.71293545
GGGAGGT274550.014.2612013
GTTGTCG24000.014.1639526
GGAGGTC266000.014.1121874
GGCATAG188750.014.01756235
ATAGGGG195000.013.72898238
GAGCTTA201150.013.21809344
TGGCATA206700.012.80008334
CATAGGG229450.011.65862637
TGGGAGG247950.011.4908682
CGTCAGT110500.010.6853581
ATATAGC38600.010.6663996
GCATAGG252450.010.65882536
CTGGGAG308800.010.5895911
ACTCTCC360550.010.486896511
GCTCTGT359550.010.42294422
TAGGGGA356300.010.41162439
CCGCGTT26050.010.36734913
CGCGTTC25700.010.33272814
TCTCCCC366850.010.22683613
Produced by FastQC (version 0.11.5)