FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47772349
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG509970.1067500365117068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC91500.019.7719024
CAGACCG102500.018.045193
TTCGACG17150.017.4446356
GCCGGCT167900.017.0460191
GACCGCG107100.016.7659025
CCGGCTT199950.016.0838932
TTCACGC196650.015.6711567
ACCGCGT117050.015.5279586
GTTCCTA82450.015.2236215
CGTTACG14050.014.56941821
CCGCGTT124450.014.532347
CGCGTTC125100.014.3669278
GTCCGTT33850.014.3601251
TCACGCT216450.014.1233028
CCCGGAT105050.013.9465481
AACGCTA212100.013.91458623
CTCCGAT44250.013.9110251
CCTACTC90750.013.85614218
ACGCTAC213900.013.83955924
CCCGTAT30700.013.61391
Produced by FastQC (version 0.11.5)