FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50669810
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG150050.015.14951745
GGCATAG166850.013.672935535
GGGAGGT262600.013.3029953
ATAGGGG177650.013.1595938
GAGCTTA180950.012.82372944
TGGCATA192800.011.9023234
CATAGGG196450.011.69298737
GGAGGTC290350.011.3802194
GTTGTCG20300.011.30278926
GCATAGG212350.010.80688536
ACCCGGC67450.010.7440085
ACTCTCC307350.010.4836211
TAGCGTA7400.010.33710637
TGGGAGG242400.010.2794942
ATAGCTC65250.010.27755939
TCAGACG81750.09.96732
AGGGATC155000.09.962062
GGTCACT334100.09.718427
GCTCTGT338100.09.60780122
TCTCCCC343350.09.52857513
Produced by FastQC (version 0.11.5)