FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47131163
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT687970.1459692390786113No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG680950.14447977869758913No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC471710.10008452369401537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT246650.025.57077845
AGAACGA301300.020.93460344
AGACCGC302050.020.7717254
CAGACCG307350.020.4940453
CGCGTTT59100.020.0592448
GACCGCG315600.019.8511475
ACCGCGT319700.019.610246
CCGCGTT325700.019.2491077
TCACGCT213650.018.5732158
CGCGTTC276800.018.3586858
AACGCTA208550.018.27417423
ACGCTAC211300.018.07903124
TCAGACC354850.018.0424042
ATACGGA321800.017.65812933
AATACGG322800.017.61200132
TACGGAG326150.017.41542234
TTCGGCG83200.017.27680444
CGCTACC221450.017.25039325
CTCAGAC397500.017.2503031
AAAACGC224750.016.99335321
Produced by FastQC (version 0.11.5)