FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47131163
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG787420.16706992780975932No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA549850.1166637878212341No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG522770.11091812014059574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG227850.015.99902145
ACCCGGC103400.015.9543885
GGCATAG251150.014.54805335
GTTGTCG24300.014.45367426
GAGCTTA265450.013.85885744
GTCACTC367600.013.8536368
GAGGTCA379200.013.7872485
GGTCACT372450.013.6798297
TGGCATA269050.013.61047134
ACACGGA122550.013.57809816
ATAGCTC122050.013.36452839
ACTCTCC387700.013.34041111
ATAGGGG277650.013.32156638
GGGAGGT422500.013.2384343
AGGTCAC394900.013.1810266
TCACCCG134600.013.1101583
GGAGGTC401500.012.971574
CGGACAC132950.012.90552513
CCGGACA135000.012.85927812
TCACTCT417350.012.556999
Produced by FastQC (version 0.11.5)