FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47952831
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG40150.016.1953874
AGACCGC78200.016.1124044
CCCGTAT34400.015.8420721
CGTACGC42950.015.611055
GTCCGTT39500.015.3359351
CAGACCG83900.015.2591143
CCCCCCT47600.014.9024831
CTCCGAT47450.014.7597581
TTCGACG16700.014.6850516
CCCGGAT120550.014.5894491
CGTTACG13850.014.45791521
GTTCCTA73150.013.93322415
GACCGCG90450.013.9302375
TACGCCA49250.013.7050457
CTCCGTT65550.013.5699881
CTCGGTT81150.013.458861
CCGGCTT153750.013.4194472
CCGGATA78750.013.3999342
AACGCTA116500.013.36429623
AGACGCT163450.013.28391141
Produced by FastQC (version 0.11.5)